| HgCl2对斑马鱼SOD,AChE酶活性和基因相对转录量的影响 |
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| 引用本文： | 何桢,牟文,周巧巧,杨雪,张灿,胡芹芹,胡晓静,郭佳,钟秋,熊丽,刘德立. HgCl2对斑马鱼SOD,AChE酶活性和基因相对转录量的影响[J]. 生态毒理学报, 2011, 6(6): 649-654 |
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| 作者姓名： | 何桢 牟文 周巧巧 杨雪 张灿 胡芹芹 胡晓静 郭佳 钟秋 熊丽 刘德立 |
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| 作者单位： | 华中师范大学生命科学学院;湖北省遗传调控与整合生物学重点实验室,武汉,430079;湖北省襄阳市第五中学,襄阳市,441057 |
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| 基金项目： | 国家自然科学基金(30771429.31071653);教育部留学归国人员科研基金 |
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| 摘 要： | 以HgCl2为实验毒物,研究其对斑马鱼的毒性效应,经染毒后,分别在第1天和第7天取斑马鱼的肌肉测定超氧化物歧化酶(SOD)、乙酰胆碱酯酶(AChE)活性,半定量RT-PCR检测肌肉中乙酰胆碱酯酶、细胞色素P450(CYP 1A)和超氧化物歧化酶(Cu/Zn-SOD)基因的相对转录量.结果显示HgCl2对斑马鱼的毒性很强...
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| 关 键 词： | HgCl2 斑马鱼 AChE CYP 1A Cu/Zn-SOD |
| 收稿时间： | 2011-05-18 |
| 修稿时间： | 2011-07-27 |
Toxic Effects of HgCl2 on SOD and AChE of Activities and Gene Transcription of Zebrafish |
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| He Zhen,Mu Wen,Zhou Qiaoqiao,Yang Xue,Zhang Can,Hu Qinqin,Hu Xiaojing,Guo Ji,Zhong Qiu,Xiong Li and Liu Deli. Toxic Effects of HgCl2 on SOD and AChE of Activities and Gene Transcription of Zebrafish[J]. Asian Journal of Ecotoxicology, 2011, 6(6): 649-654 |
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| Authors: | He Zhen Mu Wen Zhou Qiaoqiao Yang Xue Zhang Can Hu Qinqin Hu Xiaojing Guo Ji Zhong Qiu Xiong Li Liu Deli |
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| Affiliation: | Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430079, China;Xiang Yang No.5 Middle School, Xiang Yang 441057, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430081, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430082, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430083, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430084, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430085, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430086, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430087, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430088, China;Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan 430089, China |
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| Abstract: | Acute toxicity tests(96 h) were conducted in static-renewal aquarium systems to determine the lethal toxic effect of HgCl2 on fish.Zebrafish were exposed in HgCl2 with various levels.Superoxide dismutase(SOD) and acetylcholinesterase(AChE) activities were measured after 1 and 7 d.Muscle RNA was also extracted for semi-quantitative RT-PCR determination of AChE,CYP 1A,Cu/Zn-SOD transcription amounts.It showed that HgCl2 was highly toxic on zebrafish.Specifically,the enzymatic activities and the gene transcrip... |
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| Keywords: | HgCl2 zebra fish AChE CYP 1A Cu/Zn-SOD |
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