1株低温石油烃降解菌的分类鉴定及降解特性研究

从渤海油船泄漏区域的海底泥中筛选到1株能降解柴油的低温石油烃降解菌T7-2,初步鉴定为红平红球菌(Rhodococcus erythropolis).研究表明,利用工业乙醇无机盐培养基培养液体种子是适宜的,其最适温度和pH分别为15℃和7.8,工业乙醇最适加入量为0.5％,菌体浓度为10⁸ CFU/mL.接种人工海水烃降解培养基后,通过补加氮源 (NH₄)₂SO₄ 2.64　g/L、磷源Na₂HPO₄ 2.5 g/L和酵母粉 0.015 g/L后,15℃振荡培养7　d,降解率可以达到73.2％.该菌降解烷烃的范围很广泛,C12～C36均有不同程度的降解.

Isolation and Identification of a Low Temperature Hydrocarbon-degrading Strain and Its Degradation Characteristics

A low-temperature hydrocarbon-degrading strain T7-2 was isolated from sea-mud of Bohai polluted area and identified as Rhodococcus erythropolis, which could use diesel oil as carbon source. The optimal temperature and pH for the strain utilizing ethanol was 15 degrees C and 7.8, and the optimal concentration of ethanol and the seed culture was 0.5% and 10(8) CFU/mL, respectively. Inoculated to artificial seawater which was added (NH4)2SO5 2.64 g/L, Na2HPO4 2.5 g/L and yeast extract 0.015 g/L after 7 days of culture at the temperature of 15 degrees C, the rate of degradation was 73.2%. The strain could degrade a large range of n-alkane from C12 to C36.