基于间接竞争抑制ELISA的2,2',4,4'-四溴联苯醚检测方法

针对现有的检测多溴联苯醚（PBDEs）方法中存在的样品处理繁复、样品检测耗时以及费用昂贵等不足，开展了针对此类物质中代表性化合物2，2'，4，4'-四溴联苯醚（BDE-47）的间接竞争抑制酶联免疫吸附法（ELISA）的检测分析研究，并与气相色谱-质谱检测法、酶联免疫吸附分析试剂盒检测法进行对比分析。研究表明：间接竞争抑制ELISA法测定BDE-47的IC50值为0.11 mg·L-1，定量限为0.04 mg·L-1，检出范围为0.01~0.50 mg·L-1。该方法在满足复杂基质中微量目标物检测灵敏度及重现性要求下，具有检测更快捷、更经济实用的特点，宜适用于大批量此类环境样品的初期筛查。

Resarch of 2, 2', 4, 4'-Tetrabromodiphenyl Ether detection method based on indirect competitive inhibition enzyme-linked immunosorbent assay

The existing methods for the detection of polybrominated diphenyl ethers (PBDEs) in complex samples face challenges like high costs and long sample testing times. This study assessed the indirect competitive inhibition enzyme-linked immunosorbent assay (ELISA) of 2, 2', 4, 4'-tetrabromodiphenyl ether (BDE-47) as a representative PBDE, comparing performance with those of gas chromatography-mass spectrometry (GC-MS) detection methods and ELISA kits. For the indirect competitive inhibition ELISA method, the value of IC50 was 0.11 mg·L-1, the limit of quantification was 0.04 mg·L-1, and the detection range was 0.01-0.50 mg·L-1. Meeting the requirements of sensitivity and reproducibility of detecting trace targets in a complicated matrix, ELISA is a more efficient, economic, and practical method suitable for early screening of large quantities of environmental samples.