AOA-SBR系统脱氮除磷性能及PAOs的代谢特性

以SBR反应器为研究对象，采用人工配水，考察在厌氧/好氧/缺氧（AOA）运行方式下系统的脱氮除磷性能，并通过对排水比、好氧停留时间和进水氨氮负荷的调试，优化运行条件。在优化工况下，通过分析典型周期系统中胞内聚合物的形成与转化情况，研究AOA-SBR系统中聚磷菌（PAOs）的代谢特性。结果表明：在AOA-SBR系统中，排水比为50%，好氧停留时间为110 min，进水氨氮负荷为0.20 kg N·（kg MLSS·d）-1是系统的优化工况；厌氧段合成PHA的能量主要来自于聚磷水解，还原力大多来自于糖酵解，只有25% 来自于TCA循环；PHA和糖原是驱动缺氧段反硝化的共同碳源。

Nitrogen and phosphorus removal in AOA-SBR system and metabolic properties of PAOs

This research focused on the anaerobic/oxic/anoxic sequencing batch process (AOA-SBR system) using artificial wastewater and investigated its performance in the removal of nitrogen and phosphorus. Moreover, the operation parameters, including displacement ratio and HRT of aerobic and ammonia nitrogen load of the AOA-SBR system, were investigated. Under a stable optimum operation, the metabolic properties of phosphorus accumulating organisms (PAOs) in this system were studied by analyzing the formation and transformation of the intracellular polymer in a typical cycle. The results suggested that when the drainage ratio is 50%, the aerobic residence time is 110 min, and the influent ammonia load is 0.20 kg N·(kg MLSS·d)-1; then, the system can achieve optimum operation. In addition, it could be speculated that dominant PAOs in the anaerobic phase relied more on glycolysis than on poly-P hydrolysis for energy; the reducing equivalents for synthesizing PHA was mainly from glycolysis and only 25% originated from the TCA cycle. At the same time, the carbon source driving the anoxic denitrification was provided by PHA and glycogen.