厌氧发酵方式对乳渣废水产酸性能的影响

为了研究厌氧发酵方式对乳渣废水发酵产酸效果的影响，分别对乳渣废水采用自然型发酵、填料型发酵及中性型发酵（pH=7.0±0.2），并进行连续2个周期的厌氧反应。分析乳渣废水中可溶性化学需氧量（SCOD）、蛋白质、多糖、挥发性短链脂肪酸（SCFAs）、水解酶（蛋白酶、α-葡萄苷酶）和辅酶420、NH4+-N及PO43--P指标的变化。研究发现，中性产酸系统中水解酶活性较高，蛋白质和多糖等物质水解性能较好，使乳渣废水产酸性能最佳，最大SCFAs积累量为12 328.37 mg-COD·L-1。自然发酵产酸系统中水解酶活性最低，系统中残留大量的蛋白质和多糖等物质，最低SCFAs积累量为4 322.61 mg COD·L-1。同时发现，3个发酵系统中挥发性短链脂肪酸酸成分具有显著差别，其中自然型发酵系统乙酸积累率最大，可达69.70%，中性发酵系统丙酸积累率最大可达49.27%，填料型发酵系统正丁酸积累率最大可达38.85%。

Effect of milk residue wastewater anaerobic fermentation method on acidogenicity performance

To investigate the effects of an anaerobic fermentation method on milk-residue wastewater acidogenicity, natural, beaumontage, and mediosilicic fermentation (pH=7.0±0.2) were used with two operation cycles continuously. Different chemical and biological indicators, such as soluble chemical oxygen demand, protein, polysaccharides, short-chain fatty acids (SCFAs), hydrolase (protease, α-glycosides enzyme), coenzyme F420, NH4+-N, and PO43-P, were analyzed in the anaerobic fermentation process. The mediosilicic fermentation system had high hydrolase activity, which led to higher hydrolysis rates of protein and polysaccharides. This caused higher acidogenicity performance, and the maximum SCFA accumulation was 12 328.37 mg COD·L-1. However, the natural fermentation system had lower hydrolase activity, and large amounts of protein and polysaccharides were left, which led to lower SCFA production;the minimum value was 4 322.61 mg COD·L-1. Individual SCFAs showed significant differences between the three fermentation systems, where the maximum accumulation rate of acetic acid was 69.70% in the natural fermentation system, that of propionic acid was 49.27% in the mediosilicic fermentation system, and that of butyric acid was 38.85% in the beaumontage fermentation system.