一株异养硝化-好氧反硝化功能菌的分离鉴定及其脱氮特性

从活性污泥中筛选出一株具有异养硝化同时好氧反硝化功能菌N7，经分析鉴定为Acinetobacter calcoaceticus（醋酸钙不动杆菌）。以硫酸铵为氮源、丁二酸钠为碳源，菌株N7对NH4+-N去除率高达99.2%，TN去除率为67.1%，异养硝化效果较好。分别以硝酸钾和亚硝酸钠为氮源，在30℃，150 r·min-1下培养，其TN去除率分别为62.7%和75.0%，表明N7具有良好的好氧反硝化效果。单因素实验结果表明菌株N7的最佳反硝化条件为：温度30℃、转速230 r·min-1、C/N 35、pH 8.1。

Isolation,identification and nitrogen removal characteristics of a heterotrophic nitrification-aerobic denitrification bacterium

A heterotrophic nitrification-aerobic denitrification bacterium, N7, was isolated from activated sludge. The bacterium was identified as Acinetobacter calcoaceticus according to its morphological character, physiological, biochemical identification, and the 16S rDNA sequence analysis. With ammonium sulfate and sodium succinate as the sole nitrogen source and carbon source, respectively, the removal rate of the ammonia nitrogen of strain N7 was 99.2%; however, the removal rate of total nitrogen was only 67.1%. This result suggested that strain N7 had a satisfactory heterotrophic nitrification effect, and a superior effect on aerobic denitrification. The removal rates of total nitrogen were respectively 62.7% and 75.0%, using potassium nitrate or sodium nitrite as the sole nitrogen source, at 30℃ and 150 r·min-1. The experimental results showed that the optimum denitrifying condition was obtained at 30℃, 230 r·min-1, C/N ratio of 35, and pH of 8.1.