Biodegradation and detoxification of olive mill wastewater by selected strains of the mushroom genera Ganoderma and Pleurotus |
| |
Authors: | Ntougias Spyridon Baldrian Petr Ehaliotis Constantinos Nerud Frantisek Antoniou Theodoros Merhautová Věra Zervakis Georgios I |
| |
Affiliation: | a Democritus University of Thrace, Department of Environmental Engineering, Laboratory of Wastewater Management and Treatment Technologies, Vas. Sofias 12, 67100 Xanthi, Greece b Institute of Microbiology of the ASCR, Videnska 1083, 14220 Prague, Czech Republic c Agricultural University of Athens, Laboratory of Soils and Agricultural Chemistry, Iera Odos 75, 11855 Athens, Greece d National Agricultural Research Foundation, Institute of Kalamata, Lakonikis 87, 24100 Kalamata, Greece e Agricultural University of Athens, Department of Agricultural Biotechnology, Laboratory of General and Agricultural Microbiology, Iera Odos 75, 11855 Athens, Greece |
| |
Abstract: | Thirty-nine white-rot fungi belonging to nine species of Agaricomycotina (Basidiomycota) were initially screened for their ability to decrease olive-mill wastewater (OMW) phenolics. Four strains of Ganoderma australe, Ganoderma carnosum, Pleurotus eryngii and Pleurotus ostreatus, were selected and further examined for key-aspects of the OMW biodegradation process. Fungal growth in OMW-containing batch cultures resulted in significant decolorization (by 40-46% and 60-65% for Ganoderma and Pleurotus spp. respectively) and reduction of phenolics (by 64-67% and 74-81% for Ganoderma and Pleurotus spp. respectively). COD decrease was less pronounced (12-29%). Cress-seeds germination increased by 30-40% when OMW was treated by Pleurotus strains. Toxicity expressed as inhibition of Aliivibrio fischeri luminescence was reduced in fungal-treated OMW samples by approximately 5-15 times compared to the control. As regards the pertinent enzyme activities, laccase and Mn-independent peroxidase were detected for Ganoderma spp. during the entire incubation period. In contrast, Pleurotus spp. did not exhibit any enzyme activities at early growth stages; instead, high laccase (five times greater than those of Ganoderma spp.) and Mn peroxidases activities were determined at the end of treatment. OMW decolorization by Ganoderma strains was strongly correlated to the reduction of phenolics, whereas P. eryngii laccase activity was correlated with the effluent’s decolorization. |
| |
Keywords: | White-rot fungi Mushroom Toxicity Olive-oil mill phenolics OMW decolorization |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|