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Isolation and identification of an anti-algal compound from Artemisia annua and mechanisms of inhibitory effect on algae |
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| Authors: | Ni Lixiao Acharya Kumud Hao Xiangyang Li Shiyin |
| Institution: | a Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, MOE, School of Environment, Hohai University, 210098 Nanjing, China b Desert Research Institute, Las Vegas, NV 89119, USA c Shanxi Lu’an Engineering Investigation & Design Co. Ltd., 046204 Changzhi, China d Department of Environmental Science and Engineering, School of Geography Science, Nanjing Normal University, 210097 Nanjing, China |
| Abstract: | The goals of this work were to isolate and identify an anti-algal compound from extracts of Artemisia annua and study its mode of action on Microcystis aeruginosa. The anti-algal compound was isolated from the extracts using column chromatography and activity-guided fractionation methods. Artemisinin with strong anti-algal activity was identified by gas chromatography-mass spectrometry and 1H Nuclear Magnetic Resonance. The EC50 of artemisinin on M. aeruginosa was 3.2 mg L−1. Artemisinin decreased the soluble protein content and increased the superoxide dismutase activity and ascorbic acid content of M. aeruginosa, but exerted no effect on soluble sugar content. The results suggested the mode of action of artemisinin on algae may primarily be the increasing level of reactive oxygen species in algae cells. The results of our research could aid in the development of new anti-algal substances and lead to further study of mechanisms of inhibitory effect on algae. |
| Keywords: | Compositae Allelochemicals Artemisinin Microcystis aeruginosa Inhibitory mechanisms |
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