Cytochrome c used as a model system to probe the structural properties of benthic mucilage |
| |
Authors: | M B Fadda P Scano M Paci A M Deiana P Sarti |
| |
Institution: | (1) Institute of Biological Chemistry, University of Cagliari, Via della Pineta 77, I-09125 Cagliari, Italy, IT;(2) Department of Sciences and Chemical Technologies, University of Rome, Tor Vergata, Rome, Italy, IT;(3) Department of Animal Biology and Ecology, University of Cagliari, Italy, IT;(4) Department of Biochemical Sciences, University of Rome, La Sapienza, Rome, Italy, IT |
| |
Abstract: | Benthic mucilage, whether native or artificially fragmented to microscopic dimensions by ultrasonic treatment, was mixed
with cytochrome c, which was used as a 12 000 Da polycationic model compound. Cytochrome c binding profiles proved to depend on the aggregation state of the mucilage. The native mucus matrix binds cytochrome c quasi hyperbolically with an apparent affinity constant K = 1 × 106
M
−1. As shown by chemical modification of both the mucilage and cytochrome c, the binding expression is dependent on the availability of both the positive charges on the cytochrome c surface and the negative charges within the mucus matrix. The extent of binding is sensitive to the ionic strength of the
medium. The ultrasonic-stabilized mucus fragments display a peculiar binding profile, with an apparent low affinity, abruptly
entering into a high affinity binding region. The results suggest that, depending on the mucus to polycation ratio, a polymeric
reticulus builds up. This reticulus can accommodate molecules of at least 12 000 Da molecular weight. The results are also
discussed with respect to biological implications.
Received: 23 April 1997 / Accepted: 24 November 1997 |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|