酵母菌-细菌联合修复石油污染土壤研究

采用室内模拟方法研究热带假丝酵母菌(Candida tropicalis)和粪链球菌(Streptococcus faecalis)对石油污染土壤的联合修复，并监测修复过程中土壤脱氢酶活性、石油污染组分的变化. 结果表明:酵母菌-细菌混合菌剂在对石油烃的去除中表现出协同作用，连续培养32 d后混合菌处理组对总石油烃(TPH)的去除率达到29.9%，较单菌处理组高约50%；混合菌处理组脱氢酶活性高于单菌，并与石油烃去除率呈很好的相关性；前期低分子量烷烃降解效果明显，对于C15～C21的高分子量烷烃的降解效果主要在第32天时显现. 

Bioremediation of Petroleum-Contaminated Soil with a Microbial Mixture of Yeast and Bacteria

The effect of a microbial mixture of Candida tropicalis and Streptococcus faecalis on the remediation of petroleum-contaminated soil was evaluated in a laboratory. The changes of dehydrogenase activity of soil and petroleum constituents were monitored during the bioremediation process. The results showed that the microbial mixture showed synergistic effects in the removal of petroleum hydrocarbons. In the mixed microorganisms treatment group, 29.9% of the total petroleum hydrocarbon (TPH) was removed after 32 days of incubation, which was 50% higher than that observed in the single microorganism-treatment groups. Higher dehydrogenase activity was observed in the mixed microorganisms treatment group, and a positive correlation was obtained between dehydrogenase activity and biodegradation efficiency. Within the early stage of treatment, low molecular weight alkanes were degraded significantly, while high molecular weight components (C15-C21) were metabolized beginning from 32 days.