五氯酚(PCP)高效降解菌Psendomonas sp.CS5的研究

从曾受五氯酚(pentachlorophenol，简称PCP，下同)污染废水的活性污泥中，经驯化富集和分离筛选获得一株PCP降解菌，菌号为CS-W-98-5.在24h内该菌降解w(PCP)=400×10

STUDIES ON PSENDOMONAS SP. CS5 THAT DEGRADES HIGH CONCENTRATION OF PENTACHOROPHENOL

A pentachlorophenol degrading bacteria was isolated from sewage sludge containing PCP waste after enrichment. Main characteriseics of CS-W-98-5 strain are Gram negative,single polar flagelled,oxidase and catalase positive.It was identified as Psendomonas sp. by morphological，physiological and biochemical characteristics.It could use PCP as sole source of carbon and energy. CS-W-98-5 strain was able to degrade a higher concentration of PCP than previously reported PCP-degrading bacteria. The degradation percentage was over 90% within 24 h. pH 6.5～8.0 and 28 ℃ were the optimum conditions for growth, while the optimum conditions for degradtion were 28 ℃，pH 7.0～9.0. The strain was very sentitive to sodium chloride. CS-W-98-5 strain required multiple inoculations for effective degradation of PCP. It could also utilize 2,4,6-trichlorphenol, 2,3,4,6-tetrachlorphenol, and Cl-OPA. Natrient amendmend affected PCP degradation.Induction could also increase PCP degrading rate. The PCP-4-monooxygenase gene(pcpB gene) was analyzed for this strain and Sphingomonas sp. RA2 by PCR and hybridization. The results of hybridization verified the similarity of PCP degrading genes of these strains, but the results of PCR showed the difference exiting between these two strains. Fig 9, Tab 4, Ref 10