Molecular Evidence for a Unique Evolutionary Lineage of Endangered Sonoran Desert Fish (Genus Poeciliopsis)

Efforts to restore an endangered species in its former range should be based on a sound understanding of evolutionary relationships among remaining natural populations. In this study mitochondrial (mt) DNA diversity within and among Gila River drainage populations of the endangered Sonoran topminnow ( Poeciliopsis occidentalis ) in Arizona was compared to that from neighboring populations in Sonora, Mexico, where the species remains locally abundant. No mtDNA diversity was detected within or among samples from the Gila River basin in Arizona. But considerable variation was found within and among populations from several river systems in Sonora. Examination of mtDNA from a population that inhabits the upper reaches of the Río Yaqui in southeastern Arizona revealed substantial divergence between it and all other populations examined. We comment on the implications of this divergent population for topminnow management in Arizona and argue for more-detailed genetic and morphological studies to determine the distributional limits and specific status of this highly divergent form.