高原城市室内灰尘对人角膜上皮细胞损伤研究

收集高海拔城市昆明不同功能区室内灰尘,利用等离子发射光谱仪分析不同粒径(F1～F4:100～2000,75～100,63～75,45～63μm)灰尘中Pb,Cu,Cd,Zn,Cr,Ni,Co,As和Mn的含量,借助人工泪液提取的方法,测定目标重金属的可溶出率(生物可给性),并分析室内灰尘人工泪提取液对人角膜上皮细胞的毒...

Damage to human corneal epithelial cells caused by indoor dust in plateau city

The indoor dust samples were collected from houses, office, and dormitory in a typical high-altitude city Kunming. Each sample was separated into 4size fractions (F1~F4: 100~2000, 75~100, 63~75, 45~63μm), and the concentrations of nine heavy metals (Pb, Cu, Cd, Zn, Cr, Ni, Co, As, and Mn) in indoor dust were detected using ICP-OES. In addition, the solubility rates (bioaccessibility) of the target heavy metals in artificial tears extraction and their cytotoxicity on human corneal epithelial cells (HCECs) were determined. The results showed that Ni, Mn, and Zn in office dust were significantly higher than those in residence and dormitory dust (P<0.01), and the contents of Cd, Ni, and Zn were 39.3, 9.5 and 12.3times higher than the background values of Kunming soils. The solubility rates of Co in residence dust, As in office dust and Mn in dormitory dust were the highest at 10.6%, 16.1%, and 36%, respectively. Heavy metals were highly susceptible to accumulate in office and dormitory dust with particle size ranging from 63 to 100μm. Moreover, the typical polygonal and cobblestone appearance of HCECs became spindle shape after artificial tear extracts of office dust exposure. Cell proliferation was enhanced at 20μg/mL whereas inhibited at higher concentration (P<0.05). The elevated mRNA expression of pro-inflammatory mediators (IL-1β and TNF-α) suggested that indoor dust from Kunming may pose a risk to inducing human corneal inflammation.