三价铁对有机物存在下厌氧氨氧化脱氮的影响

考察了三价铁（2.24~7.84mg/L）存在下厌氧氨氧化系统对有机物的耐受性能，并通过16SrRNA高通量测序技术和定量PCR探究其机理.结果表明，进水COD浓度为50和100mg/L时，4个反应器的氨氮和总氮去除率均较高（>90%），三价铁的强化作用不明显；进水COD浓度继续升高（150和200mg/L），厌氧氨氧化受到抑制，三价铁的强化作用逐渐增加；COD浓度为200mg/L时，添加三价铁（7.84mg/L）可将氨氮和总氮去除率由61.3%和79.8%（对照组）提升至71.2%和84.7%.16SrRNA高通量测序技术表明，有机物存在下，污泥微生物群落结构出现变化，主要表现为厌氧氨氧化菌丰度的降低及反硝化菌群的大量增殖，进水添加三价铁可提高浮霉菌（Planctomycetes）的丰度.定量PCR结果表明，三价铁能够提高厌氧氨氧化菌16S rRNA及功能基因hzsB的丰度.

Effect of Fe3+ on nitrogen removal of Anammox in the presence of organic matter

The tolerance of Anammox to organic matter in the presence of Fe³⁺ (2.24~7.84mg/L) was investigated by running four reactors (R1control, R2 2.24mg/L Fe³⁺, R3 4.48mg/L Fe³⁺, R4 7.84mg/L Fe³⁺). The mechanism was clarified by using 16SrRNA high-throughput sequencing technology and qPCR. The results showed that high NH₄⁺-N and TN removal rates (> 90%) of all four reactors could be achieved at the influent COD concentration of 50 and 100mg/L, and the presence of Fe³⁺ did not show the obvious positive effect. As the influent COD concentration increased to 150 and 200mg/L, the performance of Anammox was inhibited and the positive effect of Fe³⁺ increased. When the influent COD concentration was 200mg/L, the addition of Fe³⁺ (7.84mg/L) increased the NH₄⁺-N and TN removals from 61.3% and 79.8% (R1) to 71.2% and 84.7%, respectively. 16SrRNA high-throughput sequencing results indicated the decrease of Anammox bacteria and the proliferation of denitrifying bacteria in the presence of organic matter. The presence of Fe³⁺ could increase the abundance of Planctomycetes. Fe³⁺ had promoting effects on the abundance of Anammox 16S rRNA and functional gene hzsB by qPCR data analysis.