稻草秸秆发酵液的抑藻效应及其机理

为有效利用农业废弃物稻草秸秆进行抑藻,本研究对不同稻草秸秆进行了特定方式的发酵,测定了发酵液对常见淡水藻类的化感效应,探讨了其中抑藻作用强的发酵液抑藻机理.结果表明:与普通稻草秸秆发酵液相比,水稻分蘖枝发酵液对铜绿微囊藻的抑制效果显著好于普通稻草秸秆发酵液(P＜0.05),作用72h水稻分蘖枝发酵液抑制率为93.21％...

Study on the algae inhibition effect and mechanism of the fermented liquid of rice straw

In order to use agricultural waste rice straws to inhibit algae growth more efficiently, this study experimented specific fermentation of different rice straws, measured the allelopathy performance of the fermentation liquids on common freshwater algae, and discussed the mechanism of fermentation liquid with strong algae inhibition. The results showed that compared with ordinary rice straw fermentation liquids, the fermentation liquid with rice tillering branch had significantly better inhibitory effect on Microcystis aeruginosa (P<0.05), and the inhibition rate after 72h treatment was 93.21%, then increased to 97.96% at 168h. With the treatment of the ordinary rice straw fermentation liquid, the corresponding inhibition rate at 120h was only 68.20%, then decreased significantly to only 27.65% at 168h. The Eh₅₀ was 14.073h for the former and 21.036h for the latter liquid. The rice tillering branch fermentation liquid had better inhibitory effects on cyanobacteria (M. aeruginosa) and green algae (Chlorella pyrenoidosa, Scenedesmus obliquus), and had the best inhibitory effect on M. aeruginosa (P<0.05). Under the stress of rice tillering branch fermentation liquid, the contents of chlorophyll a, phycocyanin (PC) and allophycocyanin (APC) of M. aeruginosa decreased, the autofluorescence value of algal cell chlorophyll decreased continuously, and the algal cell structure was destroyed. It was speculated that one of the anti-algae mechanisms of rice tillering branch fermentation liquid might use the photosynthetic system of algae cells as its attack target to inhibit the growth of algae, and ultimately destroy the cell structure and cause cell apoptosis.