碳化硼促进Psendomonas stutzeri A1501电催化固氮产氨及机制

以Pseudomonas stutzeri（P.stutzeri） A1501为模式固氮菌株、碳化硼（B₄C）为典型电子传递载体构建电催化氮还原体系，探究B₄C添加对该体系固氮产氨性能的影响.结果表明，B₄C能够显著提高P.stutzeri A1501介导的电催化氮还原体系的产氨性能.在0.4g/L投加量条件下，900℃煅烧温度下制备的B₄C（B₄C₉₀₀）的添加能够使电催化体系氨累积量提高117%，这是由于B₄C₉₀₀表面丰富的活化位点能够促进N₂吸附与活化.此外，电化学和光谱学谱图表明，B₄C₉₀₀的添加不仅能够促使P.stutzeri A1501分泌更多的氧化还原活性物质，而且驱动高活性阴极生物膜的形成，进而强化电极电子的长距离传递及利用.

Boron carbide promotes the ammonia production by electrocatalytic nitrogen fixation with Psendomonas Stutzeri A1501

Pseudomonas stutzeri (P. stutzeri) A1501was used as a model nitrogen-fixing strain and boron carbide (B₄C) was selected as a typical electron transfer carrier to construct an electrocatalytic nitrogen reduction system. The effect of B₄C addition on the ammonia production under different conditions was explored. The results showed that B₄C significantly enhanced the ammonia production of the electrocatalytic nitrogen reduction system mediated by P. stutzeri A1501. With the dosage of 0.4g/L, the addition of B₄C (B₄C₉₀₀) prepared at 900℃ calcination temperature improved the ammonia yield by 117%. This was because the abundant activation sites on the surface of B₄C₉₀₀ promote N₂ adsorption and activation. In addition, the electrochemical and spectroscopic spectrograms showed that the addition of B₄C₉₀₀ not only triggered P. stutzeri A1501 to secrete more redox active substances, but also drove the construction of highly active cathode biofilms, thereby enhancing the long-distance transfer and utilization of electrode electrons.