氧化还原介体强化厌氧活性污泥发酵产氢特征

为提高厌氧污泥的发酵产氢能力,采用间歇培养方式考察了氧化还原介体(ROMs)对厌氧污泥发酵葡萄糖产氢效能的影响,并通过川umina MiSeq测序揭示了ROMs对微生物群落的影响.结果 表明,在发酵液体积为100mL及葡萄糖初始浓度500mg/L条件下,对照的累计产氢量和最大产氢速率(Rmax)分别为11.0mL和0....

Improving fermentative hydrogen production of anaerobic sludge by redox mediators

To improve the hydrogen production activity of anaerobic sludge, the effects of redox mediators (ROMs) on the hydrogen production efficiency of anaerobic sludge using glucose as substrate were investigated by batch culture. The microbial community structure was revealed by Illumina MiSeq sequencing. The results showed that the cumulative hydrogen production and maximum hydrogen production rate (R_max) of the control were respectively 11.0mL and 0.28mL/h under conditions of the fermentative volume of 100mL and the initial glucose concentration of 500mg/L. When humic acid and anthraquinone-2-sulfonic acid (AQS) as ROMs were added to the system, the hydrogen production capacity of anaerobic sludge was significantly improved. The cumulative hydrogen production was higher than the control by 56.4% and 13.6%, respectively. While R_max was increased by 53.6% and 10.7%, respectively. On the contrary, the hydrogen production capacity of anaerobic sludge was inhibited when graphene oxide (GO), hennaquinone and anthraquinone-2,6-disulfonic acid (AQDS) were used as ROMs. Illumina MiSeq sequencing revealed that the dominant microbial groups from each sample were different. Thermomarinilinea, Longilinea, Defluviimonas, Gimesia and Planctomicrobium were the dominant genera in control. The major genera were shifted to Thermomarinilinea, Longilinea and Ottowia in humic acid, AQS and hennaquinone fermentation systems. While Thermomarinilinea, Longilinea, Ottowia and Defluviimonas became the predominant genera in AQDS and GO fermentation systems. Redundancy analysis indicated that hydrogen production was positively correlated with Longilinea, Macellibacteroides, Trichococcus, Thioflavicoccus and Ottowia, whereas negatively correlated with Defluviimonas, Pirellula, Gimesia and Ignavibacterium.