生活污水对蝌蚪红细胞微核及核异常的诱导

利用青蛙蝌蚪红细胞微核试验,检测了城市生活污水处理厂各级处理工艺出水的诱变活性。在l6d生话污水处理的实验中,青蛙蝌蚪红细胞微核细胞率及核异常率2d后就呈现统计上的显著增加,并随暴露时间的延长而增高,第8天达到最大值。在不同浓度混合污水暴露实验中,蝌蚪红细胞微核细胞率和核异常率呈现明显的剂量依赖性增加。实验结果表明:城镇生活污水具有较强的诱变活性,一级和二级处理工艺出水也具有一定的诱变活性。现有污水处理工艺有待于进一步改进。

The Effects of Domestic Sewage on the Micronucleus and Abnormal Nucleus of Red Cell of Tadpoles

In this study,the mutagenic activity of influent, primary effluent and secondary effluent in Wuhan Sewage Treatment Plant (STP) were monitored by a micronucleus test using the erythrocytes of the tadpoles of the frog (Rana nigromaculata Hallowel1). In an experiment in which the tadpoles were exposed to domestic sewage for 16 days,the tadpole red blood corpuscles nuclear cell rates and core unusual cell rates showed a significant elevation after 2 days of exposure,and then increased over time to a maximum after 8 days.In another experiment in which the tadpoles were exposed to mixed sewage of different concentrations, the tadpole red blood corpuscles nuclear cell rates and core unusual cell rates showed significant dose-dependent increases. The results indicated that both domestic and mixed-sewage had strong mutagenic activity. And the primary effluent and secondary effluent have mutagenic activity too. The existing technology of treatment in STP should be improved.