牛粪好氧堆肥发酵微生物群落结构演替与环境因子和腐熟度的相关性

为了解好氧堆肥发酵过程中微生物群落结构变化规律和腐熟度生物学指标研究,以 自然发酵牛粪为研究材料,利用高通量测序技术分析细菌群落结构在发酵不同阶段、层次上的动态变化与环境因子和腐熟度的相互关系.结果显示:堆体上层温度、碳氮比和种子发芽指数指标优于下层;整个堆肥发酵过程中,细菌丰度和多样性指数呈先升高后降低的趋势;在门水...

CORRELATION BETWEEN THE SUCCESSION OF MICROBIAL COMMUNITY STRUCTURE AND ENVIRONMENTAL FACTORS AND MATURITY OF CATTLE MANURE AEROBIC COMPOSTING

To understand the change of microbial community structure in the process of aerobic composting fermentation and study the biological index of ripening degree, in the trial we took cow dung as the samples, and by high-throughput sequencing, the correlation between the dynamic change of microbial community structure and environmental factors and maturity at different stages and in high and low profiles was analyzed. The results showed that the temperature, C/N and seed germination index condition was better in the upper layer than the lower layer. For the whole composting period, the bacterial abundance and diversity showed a trend of first increasing and then decreasing; the bacterial community structure was at the phylum level that Firmicutes dominated the entire composting process; at the genus level, in the heating period the dominant species was unidentified_Rhizobiaceae, in high temperature period the dominant species was Bacillus, in cooling period the dominant species wsa Nocardiopsis; Spearman correlation analysis showed that there was a very significant positive correlation(P<0.01) between Bacillus and organic matters, C/N, water content and temperature; canonical correlation analysis showed that Nocardiopsis could be used as an indicator microorganism, and OM was the prominent influencing factor; the analysis of the fitted regression equation showed that there was a strong correlation between the Chao1 index and the maturity index(R2=0.986).