复合人工湿地系统对低污染水总氮的净化效果及其微生物群落结构特征

为了解复合人工湿地系统对低污染水中总氮的处理效果及微生物群落结构特征,以表流-潜流-沉水植物塘构建的复合人工湿地系统为研究对象,研究该系统对低污染水中TN、NH4+-N、NO3--N、NO-2-N的去除效果,并采用高通量测序揭示各级单元微生物群落结构与差异性.结果表明:复合人工湿地系统在0.12～0.24 m3/(m2...

PURIFICATION EFFECT OF TOTAL NITROGEN IN LOW-POLLUTED WATER BY A COMPOSITE CONSTRUCTED WETLAND SYSTEM AND ITS MICROBIAL COMMUNITY STRUCTURE

To understand the treatment effect of total nitrogen in low-polluted wastewater by composite constructed wetland system and the characteristics of microbial community structure in the constructed wetland consisted of surface flow-subsurface flow-submerged plant pond, we researched the removal rates of TN, NH₄+-N, NO₃--N and NO₂--N in the wastewater by the system. High throughput sequencing method was applied to reveal the microbial community structure and diversity in the units at all levels. The results showed that the effluent of the composite constructed wetland system reached the class Ⅳ of Environmental Quality Standard for Surface Water under the hydraulic load of 0.12~0.24 m3/(m2 · d), and had a good removal effect on pollutants. And the removal rate of TN, NH₄+-N was 62.35%~76.86% and 36.94%~74.68%. The main denitrification microorganisms in each unit showed great differences. At the genus level, the main denitrification microorganisms in the surface flow wetland were Nitrospira, 996-1 and Hyphomicrobium. The main denitrification bacteria groups in the subsurface flow wetland were Cupriavidus and Acinetobacter. The main denitrification microorganisms in the submerged plant pond were Methylotenera and Methylophilus. The results indicated that all the units contributed to the removal of total nitrogen but there were differences between them.