Acute toxicity evaluation of triazophos,deltamethrin and their combination on earthworm,Eudrilus eugeniae and its impact on AChE activity |
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Authors: | Shikha Singh Rishikesh K. Tiwari |
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Affiliation: | Biochemistry Laboratory, Department of Zoology, University of Allahabad, Allahabad, India |
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Abstract: | The acute toxicity of three formula grade pesticides namely, triazophos (an organophosphate, OP), deltamethrin (a pyrethroid) and combined pesticide (triazophos?+?deltamethrin) was determined in earthworm, Eudrilus eugeniae. They were exposed to different concentrations of these pesticides for 48 h by paper contact toxicity method. The LC50 values for triazophos, deltamethrin and of combination were determined as 0.076, 0.031 and 0.065?μg/cm2, respectively. To study the sublethal effect of these pesticides, E. eugeniae were exposed to 5% and 10% of LC50 of pesticides for 48 h. Morphological alterations such as coiling, clitellar swelling, mucus release and bleeding followed by body segmentation were observed in exposed earthworm. Acetylcholinesterase (AChE) activity assayed in different regions of body segment exhibited a significant (p?0.05) decrease in its activity particularly in the pre-clitellar region as compared to other regions. The altered behavioural responses in pesticides exposed earthworms would have been due to decline in AChE activity of the nervous system. |
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Keywords: | AChE activity behavioural changes combined pesticide Deltamethrin Eudrilus eugeniae Triazophos |
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