Determination of relative assay response factors for toxic chlorinated and brominated dioxins/furans using an enzyme immunoassay (EIA) and a chemically-activated luciferase gene expression cell bioassay (CALUX) |
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Authors: | Fatin Samara Brian K Gullett Robert O Harrison Andrew Chu George C Clark |
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Institution: | 1. U.S. Environmental Protection Agency, Office of Research and Development, National Risk Management Research Laboratory (E305-01), 109 T.W. Alexander Drive, Research Triangle Park, NC 27711, United States;2. Cape Technologies, 120 Thadeus St., Unit 2, South Portland, ME 04106, United States;3. Xenobiotic Detections Systems Inc., 1601 East Geer Street, Suite S, Durham, NC 27704, United States |
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Abstract: | Determination of toxic activity requires knowledge of both the concentration and toxicity to evaluate the risk for adverse human health and environmental effects. A chemically-activated luciferase gene expression cell bioassay system (CALUX) and an antibody-based method enzyme immunoassay (EIA) were used to detect the dioxin-like response of several polybrominated, polychlorinated, and polybrominated/chlorinated dibenzo-p-dioxins/furans (PBDDs/Fs, PCDDs/Fs, and PBCDDs/Fs, respectively). It has been suggested that the biological activity of the brominated and mixed bromo/chloro compounds is similar to their chlorinated analogues (measured by binding to the Ah receptor). PBDD/F, PCDD/F, and PBCDD/F laboratory standards exhibited biological activity ranging over three orders of magnitude. The highest relative potency (REP) values from CALUX analysis, when compared to 2,3,7,8-TCDD, were 2,3,7,8-TBDD at 0.99 (± 0.07), 1,2,3,7,8-PeCDD at 0.69, and 2-Br-3,7,8-TriCDD at 0.72 (± 0.02). Cross-reactivities were calculated using EIA for several PBDDs/Fs and PBCDDs. The highest percent cross-reactivity was found for 2,3,7,8-TBDD at 138 (± 34%), and 2,3,7-TriBDD at 84 (± 36%). |
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