| Identification of Tsuga Germplasm by Morphological Characters and RAPD Markers |
| |
| 引用本文： | ROH Mark S DE BENEDETTI Laura JOUNG Young Hee LEE Nam Sook. Identification of Tsuga Germplasm by Morphological Characters and RAPD Markers[J]. 应用与环境生物学报, 2007, 13(2): 145-151 |
| |
| 作者姓名： | ROH Mark S DE BENEDETTI Laura JOUNG Young Hee LEE Nam Sook |
| |
| 作者单位： | [1]US Department of Agriculture, Agricultural Research Service, National Arboretum, Floral and Nursery Plants Research Unit, Beltsville, MD 20705, USA [2]Istituto Sperimentale per la Floricoltura, Sanremo, Imperia, Italy [3]Chonnam National University, School of Biological Sciences and Technology, Gwangju, Korea [4]Ewha Womans University, Department of Life Sciences, Seoul, Korea |
| |
| 摘 要： | Germplasm collection is important to preserve and maximize genetic diversity for germplasm conservation. Tsuga dumosa （ D. Don） Eichler in Engler ＆ Prantl. and T. chinensis var. forrestii （Downie） Silba germplasm was collected from three localities in China： Mt. Yulong, Wenfeng Temple and Mt. Dishiergu, Yunnan Province. Accessions were identified based on morphological characters and RAPD markers. The shapes of the apices and margins of needles were examined, and the length and width of needles, cones and seeds from accessions of mature plants were used to compare the morphological differences and to identify the germplasm. Molecular markers generated by randomly amplified polymorphic DNA （RAPD） were also used to characterize the taxa. Although the clustering based on RAPD markers was inconsistent with the morphological characters of the needles, based on the overall morphological characters and on RAPD markers, the accessions from Mt. Yulong and Wenfeng Temple were identified as T. chinertsis var. forrestii, and those from Mt. Dishiergu identified as T. dumosa. Taxonomic identification of the accessions was made based on morphology and by RAPD markers concurred. The results indicate that the shapes of the apices and margins of needles particularly from young plants could not be used as a possible key to identify T. dumosa and T. chinertsis var. forrestii. Fig 6, Tab 3, Ref24
|
| 关 键 词： | 形态特征 RAPD分子标记 种质鉴定 云南铁杉 华铁杉 |
| 收稿时间： | 2006-12-08 |
| 修稿时间： | 2006-12-26 |
Identification of Tsuga Germplasm by Morphological Characters and RAPD Markers |
| |
| ROH Mark S,DE BENEDETTI Laura,JOUNG Young Hee,LEE Nam Sook. Identification of Tsuga Germplasm by Morphological Characters and RAPD Markers[J]. Chinese Journal of Applied and Environmental Biology, 2007, 13(2): 145-151 |
| |
| Authors: | ROH Mark S DE BENEDETTI Laura JOUNG Young Hee LEE Nam Sook |
| |
| Affiliation: | ROH Mark S(US Department of Agriculture, Agricultural Research Service, National Arboretum, Floral and Nursery Plants Research Unit,Beltsville, MD 20705, USA)；DE BENEDETTI Laura(Istituto Sperimentale per la Floricoltura, Sanremo, Imperia, Italy)；JOUNG Young Hee(Chonnam National University, School of Biological Sciences and Technology, Gwangju, Korea)；LEE Nam Sook(Ewha Womans University, Department of Life Sciences, Seoul, Korea)； |
| |
| Abstract: | Germplasm collection is important to preserve and maximize genetic diversity for germplasm conservation.Tsuga dumosa(D.Don)Eichler in Engler & Prantl.and T.chinensis var.forrestii(Downie)Silba germplasm was collected from three localities in China:Mt.Yulong,Wenfeng Temple and Mt.Dishiergu,Yunnan Province.Accessions were identified based on morphological characters and RAPD markers.The shapes of the apices and margins of needles were examined,and the length and width of needles,cones and seeds from accessions of mature plants were used to compare the morphological differences and to identify the germplasm.Molecular markers generated by randomly amplified polymorphic DNA(RAPD)were also used to characterize the taxa.Although the clustering based on RAPD markers was inconsistent with the morphological characters of the needles,based on the overall morphological characters and on RAPD markers,the accessions from Mt.Yulong and Wenfeng Temple were identified as T.chinensis var.forrestii,and those from Mt.Dishiergu identified as T.dumosa.Taxonomic identification of the accessions was made based on morphology and by RAPD markers concurred.The results indicate that the shapes of the apices and margins of needles particularly from young plants could not be used as a possible key to identify T.dumosa and T.chinensis var.forrestii.Fig 6,Tab 3,Ref 24 |
| |
| Keywords: | Identification of germplasm Tsuga dumosa Tsuga chinensis var. forrestii morphological characters RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
