微生物法降解药渣中残留四环素的试验研究

发酵法生产四环素过程中会产出大量废弃药渣,其中含有丰富的营养物质,但同时也残留一定量未提取完的四环素,从而限制其资源化利用。研究筛选到一株对药渣中残留四环具有高效降解作用的优势菌株,经16S rDNA鉴定为无丙二酸柠檬酸杆菌(Citrobacteramalonaticus)。该菌株在35℃、初始pH 5.5、装液量为50 mL、接种量为5%、转速为150 r/min条件下,处理四环素药渣72 h,药渣中残留四环素的降解率为86.1%。

Experimental Study on Microbial Degradation of Tetracycline Residues in Antibiotic Waste

Tetracycline production by means of fermentation generated masses of pharmaceutical solid waste which though contains rich nutrition can not be reclaimed and utilized due to the tetracycline residues it contains.In this paper,a tetracycline-degrading strain was isolated and identified by 16S rDNA as Citrobacter amalonaticus,and the degradation experiment results indicated that the antibiotic waste containing residual tetracycline was treated with C.amalonaticus for 108 h under the conditions of temperature 35 ℃,pH value 5.5,liquid volume 50 mL,incubation amount 5% and rotation speed 150 r/min.