| 应用小鼠淋巴细胞彗星试验检测地下水遗传毒性 |
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| 引用本文： | 刘瑞祥,边高鹏,兰婷婷,孙学茹,贾慧珍,任嘉红. 应用小鼠淋巴细胞彗星试验检测地下水遗传毒性[J]. 环境监测管理与技术, 2017, 29(3): 45-48 |
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| 作者姓名： | 刘瑞祥 边高鹏 兰婷婷 孙学茹 贾慧珍 任嘉红 |
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| 作者单位： | 长治学院生物科学与技术系，太行山生态与环境研究所,长治学院生物科学与技术系，太行山生态与环境研究所,长治学院生物科学与技术系,长治学院生物科学与技术系,长治学院生物科学与技术系,长治学院生物科学与技术系，太行山生态与环境研究所 |
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| 基金项目： | 山西省高等学校大学生创新创业训练重点基金资助项目（No.2015426）；地方高校国家级大学生创新创业训练计划基金资助项目（No.201510122001） |
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| 摘 要： | 应用小鼠淋巴细胞彗星试验对长治市5个点位地下水水质作遗传毒性研究。结果表明,各水样有机浓集物均可对小鼠淋巴细胞DNA产生不同程度的遗传损伤,随着剂量的增加,Olive尾矩与阴性对照组(0.72±0.09)相比均有显著性差异(P0.05,P0.01)。在试验浓度范围内,以彗星尾部DNA含量、彗星尾长、尾矩和Olive尾矩所指示的DNA损伤程度随剂量增大而逐渐增加,存在显著的剂量-效应关系,其相关系数0.815。
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| 关 键 词： | 遗传毒性；小鼠淋巴细胞； DNA损伤；彗星试验；地下水 |
Genotoxicity Evaluation of Ground Water using the Comet Assay with Mouse Lymphocytes |
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| Abstract: | In this paper, the genotoxicity of water quality in ground water were investigated with the comet assay in Changzhi. The results showed that different degrees of DNA damage in mouse lymphocytes was induced after exposure to the organic extracts from all water samples, and the significant differences of DNA damage in Olive tail moment (OTM) with dose increases were shown between the sample groups and the solvent control groups. In the test concentration range, the extent of DNA damage in the comet tail percentage of DNA (TD), tail length (TL), tail moment (TM) and Olive tail moment indicated gradually increased with dose increases. There was a significant dose response relationship, the correlation coefficients is over 0.815. |
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| Keywords: | Genotoxicity Mouse lymphocytes DNA damage Comet assay Ground water |
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