复合碳源填料反硝化脱氮及微生物群落特性

为探讨低碳氮比污水厂尾水的深度脱氮技术,以碱处理玉米芯、零价铁和活性炭组成的复合碳源作为填料,考察反硝化生物脱氮滤柱的运行效果,并借助Miseq高通量测序技术对滤柱生物膜的微生物群落组成和结构进行解析.结果表明,复合碳源填料进行反硝化脱氮时,能有效的被微生物利用并获得较高的TN去除率.在温度为28℃左右,反硝化滤柱进水NO3--N浓度为20~30mg/L、HRT=7.7h时,TN去除率可达到95%以上,出水TOC在15mg/L左右;微生物在属水平进行聚类分析结果表明,生物膜中与反硝化作用有关的菌属和与纤维素降解有关的菌属分别占已知菌属的40.35%和29.04%.因此,污泥中反硝化作用菌属和纤维素降解有关的菌属的大量存在,为复合碳源填料高效反硝化作用提供了可能.

Denitrification of composite carbon filler and character of microbial community

To study advanced denitrogenation of low C/N ratio effluent from municipal wastewater treatment plant, the composite carbon filler composed of corncob pretreated by alkali, zero-valent iron and activated carbon was made. The performance of composite carbon filler denitrification system was investigated and Miseq high-throughput was also applied to study the microbial community structures. Composite media in denitrification could be effectively used and achieve a higher total nitrogen (TN) removal rate. The TN removal efficiency of the denitrification reactor could be above 95% and the concentration of TOC in the effluent was about 15mg/L when the temperature was about 28℃, the Hydraulic Retention Time (HRT) was 7.7h and the inflow NO3--N was about 20~30mg/L. The analysis revealed that at the genus level the genera related to denitrifying and degradation of cellulose, was accounted for 40.35% and 29.04% which were related to known bacteria, respectively. So,the masses of denitrifying bacteria and cellulolytic bacterium in the sludge made it feasible to denitrify of composite carbon filler efficiently.