典型生活垃圾填埋场覆盖土微生物群落分析

采用第二代高通量测序技术Illumina MiSeq对典型生活垃圾填埋场覆盖土样(山东莱芜,SD;广东深圳,GD;上海老港,SH;重庆长生桥,CQ)进行16S rDNA V3~V4区高通量测序,并分析了Alpha多样性、物种组成和丰度、菌群结构及环境因子对群落结构的影响.结果表明:取自垃圾填埋场GD土样的物种种类多于其他土样,GD、SD、SH、CQ土样的Shannon指数分别为5.52±0.026、4.76±0.030、4.89±0.037、3.43±0.027;所有覆盖土样的优势菌为Alphaproteobacteria(α-变形杆菌纲)和Betaproteobacteria(β-变形杆菌纲),所占比例范围分别为12.67%~25.54%,14.35%~18.88%;SD、GD和SH三种覆盖土样的优势菌为Sphingomonas(鞘氨醇单胞菌属),分别占7.25%、10.67%、11.30%; Deltaproteobacteria(德耳塔变形杆菌纲)和Gammaproteobacteria(γ-变形菌杆纲)的相对丰度分别与TN(r=1.00,P<0.001)和TP(r=1.00,P<0.001)呈正相关关系,且结合RDA图,TN、TP和OM含量可能是SD土样区别于其他土样群落组成的主要因素.

Analysis of bacterial community composition in landfill cover soil

In this study, the bacterial community composition in 4geographically different location (Shandong Laiwu, SD; Guangdong Shenzhen, GD; Shanghai Laogang, SH; Chongqing Changshengqiao, CQ) was investigated by Illumina MiSeq sequencing targeting V3~V4region of 16S rDNA gene and the link between the bacterial community composition and environmental parameters was analyzed. Results showed that representatives of Alphaproteobacteria and Betaproteobacteria dominated at all 4cover soils, ranging from 12.67%~25.54% and 14.35%~18.88% of the total abundance, respectively. Cover soil of GD had higher bacterial diversity than the others, which suggested by Shannon index (5.52±0.026 Vs 4.76±0.030 of SD, 4.89±0.037 of SH, and 3.43±0.027 of CQ). Genus Sphingomonas dominated at cover soils of SD、GD and SH, accounting for 7.25%、10.67%、11.30% of the total abundance, respectively. Person correlation suggested that groups of Deltaproteobacteria and Gammaproteobacteria highly correlated to total nitrogen (TN) (r=1.00, P<0.001) and total phosphorus (TP) (r=1.00, P<0.001). Redundancy analysis (RDA) further indicated that TN、TP and organic matter (OM) are the important factors in shaping the bacterial community structure.