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Origin of the Henze solution/precipitate from morula cells of the blood of the ascidian Phallusia mammillata
Authors:Geoffrey Nette  S Scippa  Mario de Vincentiis
Institution:(1) Department of Genetics, General and Molecular Biology, Faculty of Science, University of Naples, via Mezzocannone 8, 80134 Naples, Italy e-mail: scippa@biol.dgbm.unina.it Tel.: +39-81-7903411 Fax: +39-81-5527950, IT
Abstract: The "Henze solution", derived originally from the aqueous extraction of pelleted whole blood from the ascidian Phallusia mammillata, was examined using spectral studies. The aqueous extraction of fractionated blood cells including compartment cells, signet ring cells, and morula cells obtained using cell separation techniques were also examined. It was found that this Henze solution, and the Henze precipitate itself derived from this solution, emanated solely from the morula cells. Furthermore, it was found that this solution is formed independently of the vanadium metal ions otherwise associated with the vanadocytes. Observation of the Henze precipitate by light microscopy shows that this material partially forms crystallites or microglasses. Received: 27 July 1999 / Accepted in revised form: 28 February 2000
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