| 家蝇抗菌蛋白的分离纯化及部分性质 |
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| 引用本文： | 柏鸣,周立. 家蝇抗菌蛋白的分离纯化及部分性质[J]. 应用与环境生物学报, 2001, 7(6): 568-571 |
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| 作者姓名： | 柏鸣 周立 |
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| 作者单位： | 四川大学生命科学学院, |
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| 摘 要： | 通过体壁损伤诱导家蝇（Musca domestica)幼虫产生免疫血淋巴，经沸水浴热变性、减压蒸馏浓缩、Sephadex G-15柱脱盐、CM-Sepharose离子交换层析、Sephadex G-50凝胶过滤等步骤，分离得到一种具抗菌活性的蛋白质，并达电泳纯，结果表明，分离物不具血细胞凝集活性，也不是溶菌酶，而是一种未见报道的抗菌蛋白，经SDS-PAGE测得，该抗菌蛋白Mr=12600,等电点为9.8。
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| 关 键 词： | 家蝇 抗菌蛋白 分离纯化 性质 |
| 修稿时间： | 2001-01-19 |
PURIFICATION OF A KIND OF ANTIBACTERIAL PROTEIN FROM MUSCA DOMESTICA (HOUSE FLY) LARVAE |
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| Abstract: | The immunized hemolymph was produced from Musca domestica (house fly) larvae by the inducement of injury. A kind of protein with antibacterial activity was purified by means of boiled water bath, Sephadex G-15 chromatography, CM-Sepharose ion-exchange chromatography and Sephadex G-50 gel filtration. The purified protein was not lectin and lysozyme, but a kind of unknown antibacterial protein. Its M r was 12 600 by SDS-PAGE determination and its isoelectric point 9.8. Fig 7, Ref 11 |
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| Keywords: | Musca domestica (house fly) antibacterial protein purification |
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