比值-导数光谱法快速测定香草醛

文章用比值—导数光谱法测定香草醛的含量。实验结果表明,香草醛木素溶液在pH≤7时香草醛在230 nm处有吸收,在pH>7时在248 nm处有吸收。根据不同pH值和不同浓度条件下香草醛木素溶液的比值-导数光谱,说明在230 nm处的光谱信号在pH≤7不受pH值影响,248 nm处的光谱信号在pH>7不受pH值影响。在230 nm和248 nm处的光谱信号对浓度的变化较为敏感,在此两处吸收值可用于香草醛的测定。

Rapidly Determination of Vanillin by Ratio-derivative Spectrophotometry

Ratio-derivative spectrometry was used for determination of vanillin content.Analysis results indicated that when pH ≤7 vanillin lignin has absorption at 230 nm and when pH>7 vanillin lignin has absorption at 248 nm.Ratio derivative spectrum of vanillin under different pH and different concentrations indicated that when pH≤7 the spectrum at 230 nm would not suffer from pH effect,when pH>7 the spectrum at 248 nm would not suffer from pH effect.The signals at 230 nm and 248 nm in the spectrum are more sensitive to the change of concentrations,thus the two values can be used for the determination of absorption.