Cd2+胁迫对螺旋藻生长、光谱特性及藻胆蛋白质量浓度的影响

Cd2 是造成环境污染的重金属元素之一,如何治理Cd2 污染一直是一个难题。螺旋藻(Spirulina)是一种营光合自养的蓝藻,由于其突出的营养和保健价值而被誉为21世纪最优秀的食品。高浓度的某些重金属,如Cu2 ,Mn2 ,Zn2 等会抑制螺旋藻的生长并在藻细胞内富集,但有关Cd2 对螺旋藻的影响则未见相关报道。采用室内培养法,研究了不同质量浓度(0~25mg/L)Cd2 对钝顶螺旋藻突变株(SP-Dz)的生长、光谱特性及藻胆蛋白质量浓度的影响。结果表明,不同质量浓度的Cd2 处理均会抑制螺旋藻的生长,随着Cd2 质量浓度的增加,抑制作用增强。螺旋藻藻细胞有三个主要吸收峰,分别在440nm(叶绿素a在蓝紫光区的吸收峰),626nm(藻蓝蛋白的吸收峰)和676nm(别藻蓝蛋白和叶绿素a在红橙光区的吸收峰)。Cd2 处理会使藻细胞3个主要吸收峰中的两个发生红移,分别由440nm红移到448nm,676nm红移到684nm。Cd2 会使藻胆蛋白中藻蓝蛋白的吸收峰由626nm红移到630nm,而对别藻蓝蛋白在680nm的吸收峰没有明显影响。随着Cd2 质量浓度的增加,藻胆蛋白质量浓度下降。

Effects of Cd2+ on the growth, spectroscopic properties and phycobiliprotein content of spirulina platensis

Cd2 is one of the heavy metals responsible for environmental pollution. How to control the pollution caused by Cd2 is still a question need to be further addressed. Spirulina, a group of autotrophic blue algae, is named as the excellent food of 21st century because of its high nutritional value. Some heavy metal stresses inhibit the growth of Spirulina and the heavy metals could be enriched in algae cells, such as Cu2 , Mn2 , Zn2 and others. However, there are few reports about the effects of Cd2 stress on Spirulina. Being cultivated indoor, Spirulina growth are inhibited by Cd2 in different concentration (0~50 mg/L) and the inhibitory effect is linear to Cd2 concentration. The cells of Spirulina have three main absorb peaks: one is at 440 nm (the absorb peak of chlorophyll a in blue-violet light range), the next is at 626 nm (the absorb peak of phcocyanion), the third is at 676 nm (the absorb peak of allophycocyanion and chlorophyll a in red-orange light range). The treatment of Cd2 resulted in red shift of two absorb peaks of above, one peak changed from 440 nm to 448 nm, the other from 676 nm to 684 nm. The treatment of Cd2 made the absorb peak of phycocyanion changed from 626 nm to 630 nm, but there was no effects to that of allophycocyanion. The contents of phycobiliprotein in Spirulina declined with the increasing Cd2 stress.