| 响应曲面法优化苯酚降解菌Pseudomonas stutzri JFL2008固定化细胞活化培养基 |
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| 引用本文： | 袁鹏程,金秀丽,徐威. 响应曲面法优化苯酚降解菌Pseudomonas stutzri JFL2008固定化细胞活化培养基[J]. 环境科学与管理, 2010, 35(7): 62-65 |
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| 作者姓名： | 袁鹏程 金秀丽 徐威 |
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| 作者单位： | 沈阳药科大学,生命科学与生物制药学院,辽宁,沈阳,110016 |
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| 摘 要： | 以聚乙烯醇、海藻酸钠为复合载体,固定化Pseudomonas stutzri JFL2008,通过响应曲面法优化活化培养基,考察活化培养的固定化细胞降酚能力。结论：在连续完全降解1 000 mg/L苯酚过程中,活化培养比未经活化培养的固定化细胞多运行10个批次,表明响应曲面法适用于活化培养基的优化。
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| 关 键 词： | 苯酚降解 固定化细胞 活化培养 响应曲面法 |
Medium of Active Optimization for Phenol Degradatiaon by Pseudomonas Stutzri JFL2008 Using Response Surface Methodology |
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| Yuan Pengcheng,Jin Xiuli,Xu Wei. Medium of Active Optimization for Phenol Degradatiaon by Pseudomonas Stutzri JFL2008 Using Response Surface Methodology[J]. Environmental Science and Management, 2010, 35(7): 62-65 |
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| Authors: | Yuan Pengcheng Jin Xiuli Xu Wei |
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| Affiliation: | (College of Life Science and Biopharmaceutics,Shenyang Pharmaceutical University,Shenyang 110016,China) |
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| Abstract: | Pseudomonas stutzri JFL2008 was immobilized with composite carriers of polyvinyl alcohol and sodium alginate.Activation medium were optimized using Response Surface methodology,then the phenol-degrading capability of the abtivated immobilized cells was studied.Results：In the process of phenol degradation（1 000 mg/L）,comparing with non-activated,immobilized cells activated run more than 10 batches,thus indicating the suitability of response surface methodology（RSM）in optimizing activation medium was well. |
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| Keywords: | phenol degradation immobilized cells active culture response surface mothodology（RSM） |
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