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Effects of Cd(Ⅱ) and Cu(Ⅱ) on microbial characteristics in 2-chlorophenol-degradation anaerobic bioreactors
引用本文:HUANG Aiqun,CHEN Hao,CHEN Ling,DAI Yalei,ZHAO Jianfu. Effects of Cd(Ⅱ) and Cu(Ⅱ) on microbial characteristics in 2-chlorophenol-degradation anaerobic bioreactors[J]. 环境科学学报(英文版), 2008, 20(6): 745-752. DOI: 10.1016/S1001-0742(08)62122-1
作者姓名:HUANG Aiqun  CHEN Hao  CHEN Ling  DAI Yalei  ZHAO Jianfu
作者单位:HUANG Aiqun(State key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering,Tongyi University, Shanghai 200092,China) ;CHEN Hao(State key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering,Tongyi University, Shanghai 200092,China) ;CHEN Ling(State key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering,Tongyi University, Shanghai 200092,China) ;DAI Yalei(Department of Immunology, School of Basic Medical Science,Tongji University,Shanghai 200092,China) ;ZHAO Jianfu(State key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering,Tongyi University, Shanghai 200092,China) ;
基金项目:国家高技术研究发展计划(863计划)
摘    要:The effects of Cd^2+ and Cu^2+ at 300 mg/L on anaerobic microbial communities that degrade 2-cholorophenol (2-CP) were examined. Based on the polymerase chain reaction (PCR) of 16S rDNA, bacterial community diversity and archaeal community structure were analyzed with denaturing gradient gel electrophoresis (DGGE) and cloning, respectively. Degradation capabilities of the anaerobic microbial community were drastically abated and the degradation efficiency of 2-CP was reduced to 60% after shock by Cu^2+ and Cd^2+, respectively. The bacterial community structure was disturbed and the biodiversity was reduced after shock by Cu^2+ and Cd^2+ for 3 d. Some new metal-resistant microbes which could cope with the new condition appeared. The sequence analysis showed that there existed common Archaea species in control sludge and systems when treated with Cu^2+ and Cd^2+, such as Methanothrix soehngenii, Methanosaeta concilii, uncultured euryarchaeote, and so on. Both the abundance and diversity of archaeal species were altered with addition of Cd^2+ and Cu^2+ at high concentration. Although the abundance of the predominant archaeal species decreased with Cd^2+ and Cu^2+ addition for 3 d, they recovered to some extent after 10 d. The diversity of archaeal species was remarkably reduced after recovery for 10 d and the shift in archaeal composition seemed to be irreversible. The 2-CP-degradation anaerobic system was more sensitive to Cu^2+ than Cd^2+.

关 键 词:Cd^2+  Cu^2+  微生物群  多样性  老化现象
收稿时间:2007-07-14
修稿时间:2007-08-20

Effects of Cd(II) and Cu(II) on microbial characteristics in2-chlorophenol-degradation anaerobic bioreactors
HUANG Aiqun,CHEN Hao,CHEN Ling,DAI Yalei and ZHAO Jianfu. Effects of Cd(II) and Cu(II) on microbial characteristics in2-chlorophenol-degradation anaerobic bioreactors[J]. Journal of environmental sciences (China), 2008, 20(6): 745-752. DOI: 10.1016/S1001-0742(08)62122-1
Authors:HUANG Aiqun  CHEN Hao  CHEN Ling  DAI Yalei  ZHAO Jianfu
Affiliation:1. State key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering,Tongyi University, Shanghai 200092,China
2. Department of Immunology, School of Basic Medical Science,Tongji University,Shanghai 200092,China
Abstract:The effects of Cd2+ and Cu2+ at 300 mg/L on anaerobic microbial communities that degrade 2-cholorophenol (2-CP) were examined. Based on the polymerase chain reaction (PCR) of 16S rDNA, bacterial community diversity and archaeal community structure were analyzed with denaturing gradient gel electrophoresis (DGGE) and cloning, respectively. Degradation capabilities of the anaerobic microbial community were drastically abated and the degradation efficiency of 2-CP was reduced to 60% after shock by Cu2+ and Cd2+, respectively. The bacterial community structure was disturbed and the biodiversity was reduced after shock by Cu2+ and Cd2+ for 3 d. Some new metal-resistant microbes which could cope with the new condition appeared. The sequence analysis showed that there existed common Archaea species in control sludge and systems when treated with Cu2+ and Cd2+, such as Methanothrix soehngenii, Methanosaeta concilii, uncultured euryarchaeote, and so on. Both the abundance and diversity of archaeal species were altered with addition of Cd2+ and Cu2+ at high concentration. Although the abundance of the predominant archaeal species decreased with Cd2+ and Cu2+ addition for 3 d, they recovered to some extent after 10 d. The diversity of archaeal species was remarkably reduced after recovery for 10 d and the shift in archaeal composition seemed to be irreversible. The 2-CP-degradation anaerobic system was more sensitive to Cu2+ than Cd2+.
Keywords:2-cholorophenol (2-CP)  Cd2   Cu2   microbial community  diversity  Bacteria  Archaea  DGGE  cloning
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