On-line determination of respiration rates of aquatic organisms in a mono-phase oxystat at steady-state dissolved oxygen tensions |
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Authors: | N T Eriksen J J L Iversen |
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Institution: | (1) Institute of Biochemistry, Odense University, Campusvej 55, DK-5230 Odense M, Denmark, DK |
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Abstract: | On-line determination of respiration rates at steady-state dissolved oxygen tensions was performed in a thermostatted measuring
chamber equipped with a galvanic oxygen electrode. The measuring chamber is designed to completely exclude gas bubbles from
the liquid phase and is named a mono-phase oxystat. Additions of oxygenated water to maintain steady-state dissolved oxygen
tensions are controlled by a computer, and the respiration rate is determined from the rate of these additions. An external
loop with a flow cell in a spectrophotometer makes it possible to determine and control the concentration of algal cells in
the mono-phase oxystat and allowed simultaneous determination of respiration and filtration rates of filter-feeding invertebrates.
Because the system maintains steady-state conditions the experiments do not suffer from time limitations. The response time
of the system is negligible compared to the response time of other open systems.
Received: 7 November 1996 / Accepted: 6 December 1996 |
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