| 利用SPR技术测定湖水中微囊藻毒素 |
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| 引用本文： | 周宏敏,欧惠超,任鹏,张海燕,王晓萍,罗昭锋.利用SPR技术测定湖水中微囊藻毒素[J].中国环境科学,2012,32(7):1284-1287. |
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| 作者姓名： | 周宏敏 欧惠超 任鹏 张海燕 王晓萍 罗昭锋 |
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| 作者单位： | 1. 中国科学技术大学生命科学学院,合肥微尺度物质科学国家实验室,安徽合肥230027
2. 浙江大学光电信息工程学系,浙江杭州,310027 |
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| 基金项目： | 国家"863"项目，国家自然科学基金资助项目 |
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| 摘 要： | 利用表面等离子共振(SPR)技术,建立了快速定量检测湖水中微囊藻毒素LR(MC-LR)的方法.将MC-LR共价偶联到表面等离子共振芯片表面,检测了芯片的稳定性,并利用免疫竞争抑制原理构建标准曲线,对湖水样品进行了检测.结果表明:制备的芯片信号稳定,50个循环相对标准偏差(RSD)为0.62%;该方法的最低检测浓度为0.25ng/mL,可作为水体中MC-LR的分析检测方法.
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| 关 键 词： | 表面等离子共振 芯片 微囊藻毒素 免疫竞争 |
| 收稿时间： | 2011-11-03; |
A novel detection method of microcystin of lake water by surface plasmon resonance technique |
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| ZHOU Hong-min
,
OU Hui-chao
,
REN Peng
,
ZHANG Hai-yan
,
WANG Xiao-ping
,
LUO Zhao-feng.A novel detection method of microcystin of lake water by surface plasmon resonance technique[J].China Environmental Science,2012,32(7):1284-1287. |
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| Authors: | ZHOU Hong-min OU Hui-chao REN Peng ZHANG Hai-yan WANG Xiao-ping LUO Zhao-feng |
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| Institution: | 1*(1.Hefei National Laboratory for Physical Sciences at Microscale,School of Life Sciences,University of Science and Technology of China,Hefei 230027,China;2.Department of Optical Engineering,Zhejiang University,Hangzhou 310027,China) |
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| Abstract: | Amount of Microcystin-LR in water was rapidly and quantitatively detected by SPR(surface plasmon resonance) technique.After the MC-LR being immobilized on the surface of the chip,the stability of the chip was tested,and the standard curve was constructed by the principle of competitive immune inhibition.The results showed that the relative standard deviation of 50 cycles was 0.62%,which represented the stability of the immobilized chip.And the limited concentration of detection is arrived at 0.25ng/ml..For its stability and sensitivity,the SPR detection method would be applied to assay of MC-LR in the water. |
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| Keywords: | surface plasmon resonance chip microcystin immune competition |
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