纳米碳化钨对斑马鱼胚胎发育的影响

为评价纳米碳化钨的水生态毒理效应,以模式生物斑马鱼胚胎为研究对象,观察了纳米碳化钨对斑马鱼胚胎发育的影响.结果表明,<2g/L的纳米碳化钨悬液对斑马鱼胚胎的正常发育和成活没有任何影响.在3g/L的极高浓度下,纳米碳化钨处理24~96h导致31%的幼鱼出现各种发育畸形,33%的幼鱼死亡,但仍有36%的幼鱼发育和活动均正常.通过与受精后5h及30h开始纳米碳化钨暴露的实验组相比,发现受精后24h是斑马鱼胚胎对纳米碳化钨处理最敏感的时期.胚胎卵膜的人工提前去除可以削弱纳米碳化钨对斑马鱼胚胎发育的不良影响.

Developmental impact of nano-tungsten carbide in zebrafish embryos

In order to evaluate the toxicity of nano-tungsten carbide(nano-WC) in aquatic system,zebrafish embryos were used.Results showed that the low concentration of nano-WC(<2g/L) had no significant impact on the development and function of zebrafish.Under the higher concentration of nano-WC(3g/L),31% embryos developed abnormally,33% embryos died and 36% embryos were totally normal.Results showed that 24h was the most susceptive period for zebrafish embryos exposed to nano-WC compared with that of 5h and 30h.Removal of the chorion before the exposure attenuated the adverse impact of nano-WC on zebrafish embryos.