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Evaluation of ABC efflux transporters genes expression in kidney of rainbow trout (Oncorhynchus mykiss) fed with melamine and cyanuric acid diets |
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| Authors: | Benedetto Alessandro Squadrone Stefania Prearo Marino Elia Antonia Concetta Giorgi Ilaria Abete Maria Cesarina |
| Institution: | a C.Re.A.A. - National Reference Center for the Surveillance and Monitoring of Animal feed, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, 10154 Turin, Italy b Fish Diseases Laboratory, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, 10154 Turin, Italy c Ecotoxicology Laboratory, Department of Cellular and Environmental Biology, University of Perugia, 06123 Perugia, Italy |
| Abstract: | Gene expression experiments were targeted in order to monitor the ABC efflux transporters, which is potentially involved in cellular detoxification/defense. Changes in expression levels of different ABC genes in kidney of Oncorhynchus mykiss fed with melamine and melamine + cyanuric acid enriched diets were recorded in both treated groups by mRNA ΔΔCT relative quantification method. Expression profiles of eight different ABC genes basically showed low alterations in melamine group and more consistent changes in melamine + cyanuric acid treated fish, compared with own control. In the last group ABCC2 gene over expression was the more evident alteration. These results suggest that ABC efflux system could be involved in mobilization of hydrophilic molecules in the forcing condition of chronic exposure. |
| Keywords: | Melamine Cyanuric acid ABC efflux transporters Rainbow trout mRNA expression ΔΔCT method |
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