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Oxidative stress, cytoxicity, and cell mortality induced by nano-sized lead in aqueous suspensions |
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| Authors: | Cornejo-Garrido Hilda Kibanova Daria Nieto-Camacho Antonio Guzmán José Ramírez-Apan Teresa Fernández-Lomelín Pilar Garduño Maria Laura Cervini-Silva Javiera |
| Institution: | a Departamento de Procesos y Tecnología, Universidad Autónoma Metropolitana Unidad Cuajimalpa, Mexico b Posgrado en Ciencias de la Tierra, Universidad Nacional Autónoma de México, Mexico c Laboratorio de Ciencias Biologicas, Instituto de Química, Universidad Nacional Autónoma de México, Mexico d Centro de Investigación en Ciencia Aplicada y Tecnología Avanzada, Unidad Legaria, Mexico e Instituto de Geografía, Universidad Nacional Autónoma de México, Mexico f NASA Astrobiology Institute, United States g Earth Sciences Division, Lawrence Berkeley National Laboratory, United States |
| Abstract: | This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid peroxidation), cytoxicity, and cell mortality. As determined by the Thiobarbituric Acid Reactive Substances (TBARS) method, only 6 h after incubation aqueous suspensions bearing nano-sized PbO2, soluble Pb(II), and brain-homogenate only suspensions, were determined to contain as much as ca. 7, 5, and 1 nmol TBARS mg protein−1, respectively. Exposure of human cells (central nervous system, prostate, leukemia, colon, breast, lung cells) to nano-PbO2 led to cell-growth inhibition values (%) ca. ≤18.7%. Finally, as estimated by the Artemia salina test, cell mortality values were found to show high-survival larvae rates. Microscopic observations revealed that Pb particles were swallowed, but caused no mortality, however. |
| Keywords: | Water-stable Stable nanoparticles Lipid peroxidation Lead nanoparticles Biological activity |
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