|
|||||
|
|
Retention of particulate adenosine triphosphate from Mediterranean deep-sea water by glass-fibre and membrane filters |
|
| Authors: | J Gostan N Mayer-Gostan J -C Romano |
| Institution: | 1. Centre d'Etudes et de Recherches Océanologiques de Villefranche-sur-mer, Université Pierre et Marie Curie, Station Zoologique, BP 28, F-06230, Villefranche-sur-mer, France 2. Laboratoire J. Maetz, Commissariat à l'Energie Atomique, Département de Biologie, Centre d'Etudes et de Recherches Océanologiques de Villefranche-sur-mer, F-06230, Villefranche-sur-mer, France 3. Ecologie et Biochimie Microbienne du Milieu Marin, Université de Provence, 3 place Victor Hugo, F-13331, Marseille Cédex 3, France |
| Abstract: | When deep-water samples from the Mediterranean Sea were passed through glass-fibre filters (Whatman GF/F), the adenosine nucleotide concentration of the particulate material was exceptionally low. The concentration was higher when the particulate material was concentrated on SM cellulose-membrane filters with porosities of less than 1 μm. There would seem to be two reasons for this: (1) inhibition of the bioluminescent reaction; (2) an apparently lower retention of ATP by the glass-fibre filters. In order to compare results obtained with the two different types of filters, a correction factor of two can be used if measurements are made with an internal standard. This constant factor can only be applied to samples from depths greater than 300 m. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |