| 邻苯二甲酸二丁酯影响阿尔茨海默病大鼠学习记忆能力的分子机制研究 |
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| 引用本文： | 孙燕玲,郭荣洁,陈雅琦,杨艳芳,任沛瑶,武阳,马萍,吴红年,吕建国. 邻苯二甲酸二丁酯影响阿尔茨海默病大鼠学习记忆能力的分子机制研究[J]. 环境科学学报, 2021, 41(6): 2466-2473 |
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| 作者姓名： | 孙燕玲 郭荣洁 陈雅琦 杨艳芳 任沛瑶 武阳 马萍 吴红年 吕建国 |
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| 作者单位： | 湖北科技学院基础医学研究中心,环境-免疫与神经系统疾病实验室,咸宁437100;湖北科技学院五官医学院,咸宁437100;湖北科技学院临床医学院&附属第二医院,咸宁437100 |
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| 基金项目： | 湖北省教育厅科学技术研究计划指导性项目（No.B2018178）;湖北科技学院博士启动基金项目（No.2018-20XB017）;湖北省大学生创新训练计划项目（No.201810927039） |
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| 摘 要： | 为探究邻苯二甲酸二丁酯(DBP)影响阿尔茨海默病(AD)大鼠学习记忆能力的分子机制,将56只SPF级5～6周龄雌性Wistar大鼠随机平均分为7组:阴性对照组、0.25 mg·kg-1·d-1 DBP 组、2.5 mg·kg-1·d-1 DBP 组、25 mg·kg-1·d-1 DBP 组、250 mg·kg-1·d-...
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| 关 键 词： | 邻苯二甲酸二丁酯(DBP) 阿尔茨海默病(AD) 氧化应激 学习记忆能力 海马组织 |
| 收稿时间： | 2020-10-10 |
| 修稿时间： | 2020-12-10 |
Molecular mechanism of learning and memory ability affected by dibutyl phthalate in rats with Alzheimer's disease |
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| Affiliation: | Laboratory of Environment-immunological and Neurological Diseases, Research Center of Basic Medical Sciences, Hubei University of Science and Technology, Xianning 437100;Wuguan Medical College, Hubei University of Science and Technology, Xianning 437100;Clinical Medical School & Second Affiliated Hospital, Hubei University of Science and Technology, Xianning 437100 |
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| Abstract: | To investigate molecular mechanism of learning and memory ability affected by dibutyl phthalate (DBP) in rats with Alzheimer''s disease (AD), 56 female Wistar rats aged 5~6 weeks with SPF grade were randomly divided into 7 groups on average:Saline group; DBP 0.25 group; DBP 2.5 group; DBP 25 group; DBP 250 group; AD group and AD+DBP group. The results of Morris water maze were observed after continuous intragastric administration for 28 days. And the concentrations of reactive oxygen species (ROS), reduced glutathione (GSH), malondialdehyde (MDA) and 8-hydroxydeoxyguanosine (8-OHDG) in the hippocampus were monitored. The transcriptional levels of Bcl-2, Bax and Caspase-3 mRNA were analyzed by real-time fluorescence quantitative PCR (qPCR). Western blot was used to detect the protein expression levels of Bcl-2, Bax and Caspase-3. Results showed that, compared with the Saline group, the learning and memory ability of rats in the different exposure groups gradually decreased, the levels of oxidative stress increased, with the increasing concentration of DBP (p<0.05, p<0.01). Compared with the AD group, rats in the AD+DBP group showed aggravation of learning and memory impairment, up-regulation of oxidative stress level and Bcl-2/Bax/Caspase-3 signaling pathway, with statistically significant difference(p<0.05). Thus, it is concluded that DBP can up-regulate the Bcl-2/Bax/Caspase-3 signaling pathway through oxidative stress, aggravate the damage of hippocampus in rats with Alzheimer''s disease, and lead to the decline of learning and memory ability. Results of this study reveals the effect of oxidative stress on the apoptotic signaling pathway, and provide theoretical and data basis for further study on the toxicity of DBP. |
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| Keywords: | dibutyl phthalate(DBP) Alzheimer''s disease(AD) oxidative stress learning and memory ability hippocampus |
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