Studies on Biodegradation of Nicotine by Arthrobacter sp. Strain HF-2 |
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Authors: | AIDONG RUAN HANG MIN WEI ZHU |
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Affiliation: | 1. State Key Laboratory of Hydrology , Water Resource and Hydraulic Engineering Hohai University , Nanjing , P.R. China;2. College of Life Science , Zhejiang University , Hangzhou , P.R. China;3. College of Environmental Science and Engineering , Hohai University , Nanjing , China |
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Abstract: | A nicotine-degrading bacterium, strain HF-2, was isolated from tobacco waste-contaminated soil and identified as a member of Arthrobacter sp. based on morphology, physiological tests, 16S rDNA sequence and phylogenetic characteristics. At thermal denaturation test indicated that the G + C mol% of strain HF-1 was 63.5. The relationship between the growth of the isolate and the nicotine degradation suggested that strain HF-2 could utilize nicotine as sole sources of carbon, nitrogen and energy. Blue pigment was observed during the nicotine degradation by strain HF-2. The isolate grew well at 20 to 33°C, initial pH 6.5 to 8.0 and 0.5 to 2.0 g L?1 of nicotine concentration in the nicotine inorganic salt media. The maximum growth and nicotine degradation occurred at 30°C, initial pH 7.0 and 0.7 g·L?1 of nicotine concentration in media under natural incubation condition. Strain HF-2 could degrade 100% of nicotine under the optimized incubation conditions for 43 h. The concentrations of nicotine were monitored by high performance liquid chromatography. This study demonstrates Arthrobacter sp. strain HF-2 had a great ability to degrade nicotine, and it may be available for the application to the bioremediation of environments contaminated by tobacco waste. |
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Keywords: | Nicotine Biodegradation Arthrobacter sp. strain HF-2 Identification Dynamic curve. |
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