Assessment of oxidative stress due to exposure to pesticides in plasma and urine of traditional limited-resource farm workers: Formation of the DNA-adduct 8-hydroxy-2-deoxy-guanosine (8-OHdG) |
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Authors: | Avinash M. Tope Myna Panemangalore |
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Affiliation: | Nutrition and Health Program , Kentucky State University , Frankfort, Kentucky, USA |
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Abstract: | The objective of this study was to assess the risk of genotoxicity caused due to oxidative stress using plasma and urinary levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) in farm workers for six months during a growing season. Blood and urine samples were collected once a month for six months (June to November 2003) from farm workers (n = 15) and urban unexposed controls (n = 10). Plasma and urinary levels of 8-OHdG were evaluated by Enzyme Linked Immunosorbent Assay (ELISA) technique. There was no significant difference in the urinary levels of 8-OHdG between the farm workers and the control group, but there was an approximately four-fold increase in mean values of plasma 8-OHdG levels in the farm worker group (P ≤ 0.05). |
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Keywords: | Reactive oxygen species DNA adduct 8-hydroxy-2-deoxyguanosine (8-OHdG) plasma urine farm workers. |
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