Studies on the drug resistance profile of Enterococcus faecium distributed from poultry retailers to hospitals |
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| Authors: | Alya Limayem Robert Scott Donofrio Chao Zhang Edward Haller Michael G Johnson |
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| Institution: | 1. Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, Florida, USA alimayem@usf.edu;3. Applied Research Center, National Sanitary Foundation, Ann Arbor, Michigan, USA;4. Department of Cell Biology, Microbiology and Molecular Biology, University of South Florida, Tampa, Florida, USA;5. Department of Integrative Biology, University of South Florida, Tampa, Florida, USA;6. Department of Food Science and Center for Food Safety, Institute of Food Science &7. Engineering, University of Arkansas, Fayetteville, Arkansas, USA |
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| Abstract: | The multidrug resistant Enterococcus faecium (MEF) strains originating from farm animals are proliferating at a substantial pace to impact downstream food chains and could reach hospitals. This study was conducted to elucidate the drug susceptibility profile of MEF strains collected from poultry products in Ann Arbor, MI area and clinical settings from Michigan State Lab and Moffitt Cancer Center (MCC) in Florida. Presumptive positive Enterococcus isolates at species level were identified by Matrix Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF) analysis. The antibiotic susceptibility profile for both poultry and clinical strains was determined by the Thermo Scientific's Sensititre conform to the National Committee for Clinical Laboratory Standards (NCCLS) and validated via quantitative real-time PCR (qPCR) methods. Out of 50 poultry samples (Turkey: n = 30; Chicken: n = 20), 36 samples were positive for Enterococcus species from which 20.83% were identified as E. faecium. All the E. faecium isolates were multidrug resistant and displayed resistance to the last alternative drug, quinupristin/dalfopristin (QD) used to treat vancomycin resistant E. faecium (VRE) in hospitals. Results indicate the presence of MEF strains in food animals and clinical settings that are also resistant to QD. |
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| Keywords: | Multidrug resistant E faecium poultry hospitals antimicrobial susceptibility profile virginiamycin |
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