Surface-enhanced Raman spectroscopic analysis of N6-benzylaminopurine residue quantity in sprouts with gold nanoparticles |
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| Authors: | Ping Zhang Li M. Wang Tai F. Lin Xiao D. Wei Xiao Y. Liu |
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| Affiliation: | 1. College of Life Science and Bioengineering, Beijing University of Technology, Beijing, P. R. Chinazdv@bjut.edu.cn;3. College of Life Science and Bioengineering, Beijing University of Technology, Beijing, P. R. China |
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| Abstract: | ABSTRACTA rapid and quantitative method for the determination of N6-Benzylademine (N6-BA) was established through the application of surface-enhanced Raman spectroscopy (SERS). The Raman peak intensities of N6-BA at 1002 cm?1 positively correlated to N6-BA concentrations in sprout extracts. The R2 reached 0.99, and RSDs calculated below 10% at the concentration range of 0.1 ～5μg mL?1. The average recoveries were 80.0% ～ 98.2% for blank samples intentionally contaminated at differing levels of 0.04, 0.4, and 1 μg g?1. The whole procedure, including sample preparation and SERS detection, did not exceed 30 min for a set of 6 samples. This study indicates that SERS is a promising technique for rapid tracing analysis and on-site testing of N6-BA. |
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| Keywords: | N6-Benzylademine surface-enhanced Raman spectroscopy rapid detection quantitative analysis label free detection |
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