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Identification of potential biomarkers for diazinon exposure to Japanese Medaka (Oryzias latipes) using annealing control primers |
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| Authors: | Jeong-Ha Yoo Sung-Woo Shin Jong Soo Kim Chang-Bae Kim Jong-Sung Kim Sung-Cheol Koh |
| Institution: | 1. Biological Resource Center , Korea Research Institute of Bioscience and Biotechnology , Daejeon, Korea;2. Korean BioInformation Center , Korea Research Institute of Bioscience and Biotechnology , Daejeon, Korea;3. Biological Resource Center , Korea Research Institute of Bioscience and Biotechnology , Daejeon, Korea;4. Hankuk Academy of Foreign Studies , Yongin, Korea;5. Department of Chemical Bio-engineering , Kyungwon University , Sungnam, Korea;6. Division of Civil and Environmental Engineering , Korea Maritime University , Busan, Korea |
| Abstract: | A new differential display-polymerase chain reaction (PCR) method based on annealing control primers was used to screen and identify potential biomarkers from differentially expressed genes (DEGs) in medaka exposed to sub-lethal concentration of diazinon (100 ppb). Among the differentially expressed genes identified, the majority were in functional categories of protein biosynthesis, transport and metabolism according to the gene ontology classification. The differential expression of ribosomal protein genes was quantified by real time PCR. The genes encoding ribosomal proteins including L3 and S17 were selected as potential biomarkers for diazinon exposure in medaka fish. |
| Keywords: | Medaka diazinon biomarker differentially expressed genes real time PCR annealing control primers gene ontology |