首页 | 本学科首页   官方微博 | 高级检索  
     检索      


Biodegradation of nicosulfuron by the bacterium Serratia marcescens N80
Authors:Hao Zhang  Wenhui Mu  Zhiguang Hou  Xian Wu  Weiwei Zhao  Xianghui Zhang
Institution:1. College of Resource and Environment, Jilin Agricultural University , Changchun , People's Republic of China;2. College of Plant Sciences, Jilin University , Changchun , People's Republic of China
Abstract:By enrichment culturing of the sludge collected from the industrial wastewater treatment pond, we isolated a highly efficient nicosulfuron degrading bacterium Serratia marcescens N80. In liquid medium, Serratia marcescens N80 grows using nicosulfuron as the sole nitrogen source, and the optimal temperature, pH values, and inoculation for degradation are 30–35°C, 6.0–7.0, and 3.0% (v/v), respectively. With the initial concentration of 10 mg L?1, the degradation rate is 93.6% in 96 hours; as the initial concentrations are higher than 10 mg L?1, the biodegradation rates decrease as the nicosulfuron concentrations increase; when the concentration is 400 mg L?1, the degradation rate is only 53.1%. Degradation follows the pesticide degradation kinetic equation at concentrations between 5 mg L?1 and 50 mg L?1. Identification of the metabolites by the liquid chromatography/mass spectrometry (LC/MS) indicates that the degradation of nicosulfuron is achieved by breaking the sulfonylurea bridge. The strain N80 also degraded some other sulfonylurea herbicides, including ethametsulfuron, tribenuron-methyl, metsulfuron-methyl, chlorimuron-ethyl,and rimsulfuron.
Keywords:Biodegradation  nicosulfuron  Serratia marcescens  16S rDNA  phylogenetic analysis  
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号