Secretory expression of organophosphorus hydrolase OPHC2 in Yarrowia lipolytica Polg |
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Authors: | Meng Li Xiaolan Yu Fei Wang Chao Zhai Wei Shen Xianhong Yu |
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Institution: | Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, Hubei Key Laboratory of Industrial Biotechnology, College of Life Sciences, Hubei University, Wuhan, Hubei Province, P. R. China |
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Abstract: | In the present study, recombinant organophosphorus hydrolase OPHC2 was successfully produced by Yarrowia lipolytica and purified. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot analyses showed a major polypeptide band of 36 kDa. The purified enzyme was optimally active at 65°C and pH 8.5 and also displayed good thermal and pH stability using methyl parathion (O,O-dimethyl-O-4-p-nitrophenyl phosphorothioate) as a substrate. Moreover, as Y. lipolytica is a non-pathogenic, generally regarded as safe (GRAS) yeast, the cell culture supernatant can be used directly on vegetables and fruits that are contaminated by organophosphorus pesticides. |
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Keywords: | GRAS organophosphorus hydrolase organophosphorus pesticide Yarrowia lipolytica |
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