A sensitive monoclonal antibody-based enzyme-linked immunosorbent assay for chlorpyrifos residue determination in Chinese agricultural samples |
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| Authors: | Yi H Liu Jian Chen Yi R Guo Chun M Wang Xiao Liang Guo N Zhu |
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| Institution: | 1. Institute of Pesticide and Environmental Toxicology , Zhejiang University , Hangzhou, China;2. Biological Control of Pests Research Unit , Agricultural Research Service, U.S. Department of Agriculture , Stoneville, Mississippi, USA |
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| Abstract: | A monoclonal antibody-based competitive antibody-coated enzyme-linked immunosorbent assay (ELISA) was developed and optimized for determining chlorpyrifos residue in agricultural products. The IC50 and IC10 of this ELISA were 3.3 ng/mL and 0.1 ng/mL respectively. The average recoveries in six agricultural products were between 79.5% and 118.0%, with the intra-assay coefficient of variation being less than 8 %. The limit of detection for all tested products was 30 ng/g. To the best of our knowledge, this assay has the best specificity among all the published research on ELISAs for chlorpyrifos. |
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| Keywords: | Chlorpyrifos monoclonal antibody enzyme-linked immunosorbent assay (ELISA) agricultural products |
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