Determination of fumonisins in milk |
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| Authors: | PM Scott T Delgado DB Prelusky HL Trenholm JD Miller |
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| Institution: | 1. Food Research Division , Bureau of Chemical Safety, Food Directorate, Health Protection Branch,Health Canada , Ottawa, Ontario, Canada , K1A 0L2;2. Visiting scientist, Instituto Fermentaciones Industriales , Madrid, 28006, Spain;3. Centre for Food and Animal Research , Agriculture and Agri‐Food Canada , Ottawa, Ontario, K1A 0C6;4. Plant Research Centre , Agriculture and Agri‐Food Canada , Ottawa, Ontario, K1A 0C6 |
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| Abstract: | Abstract Fumonisin B1 (FB1) and fumonisin B2 (FB2) were determined in milk by liquid chromatography (LC) following immunoaffinity column cleanup. Recoveries from milk spiked with 5–50 ng each fumonisin/ml averaged 79–109%. The aminopentol hydrolysis product of FB1 (AP1) was determined by LC after cleanup on a C18solid phase phase extraction column; mean recoveries were 69–83% at spiking levels of 50–100 ng AP1/ml milk. Detection limits were of the order 3–7 ng/ml for FB1 and FB2, and 20–25 ng/ml for AP1. A stability study showed no losses of FB1 and FB2 in milk under conditions of freezing, refrigeration and boiling. A transmission study using four cows dosed with pure FB1, either orally (1.0 and 5.0 mg FB1/kg b.w.) or by i.v. injection (0.05 and 0.20 mg FB1/kg b.w.) showed no detectable residues of FB, or AP1 in the milk, with or without hydroiytic treatment with β‐glucuronidase/sulfatase to liberate any conjugates. |
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| Keywords: | fumonisin B1 fumonisin B2 aminopentol dairy cow milk liquid chromatography |
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