Dermal exposure to pesticides modifies antioxidant enzymes in tissues of rats |
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Authors: | Myna Panemangalore Frederick N Bebe |
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Institution: | 1. Nutrition and Health Program , Kentucky State University , Frankfort, KY, 40601 E-mail: mpaneman@gwmail.kysu.edu;2. Nutrition and Health Program , Kentucky State University , Frankfort, KY, 40601 |
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Abstract: | Abstract Superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities were determined in rat tissues after dermal exposure to pesticides. Two experiments were conducted in male SD rats, 190–210 g body weight. Acephate (ACP), methamidophos (MAP) and nicotine (NIC) were dissolved either individually or together in 0.25 mL of 50% ethanol, which contained: AP=12.6 or MAP 1.3 or NIC= 9.6 mg; EXP 1 ‐ individual pesticide exposure; 64 rats, 16/group; EXP 2 ‐ mixture of AP+MAP+NIC at levels of IX, 2X, 3X; 48 rats, 12/group; 0.25 mL of solution or ethanol (Controls) was applied to 25 mm2 area of shaved skin 3 times a week. Half the rats were terminated after 4 weeks and the rest after 4 weeks of stopping exposure. Single pesticides decreased erythrocyte (RBC) SOD by 17 % after exposure and in the NIC group after post exposure (P#0.05). Increasing concentrations of AP+MAP+NIC mixture elevated RBC SOD by 22 % in the 2X and 3X groups and CAT by 13 % in the 3X group (P#0.05); post exposure increased RBC SOD by 2–3 fold and CAT activity by 13 % in all 3 groups. Liver GPX increased by 30–40 % and CAT decreased by 12 % in all exposed and post exposed groups (P#0.05). The results suggest that dermal exposure to mixtures of pesticides can selectively induce SOD, CAT and GPX activities in RBC and liver. |
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Keywords: | Pesticides Superoxide dismutase catalase glutathione peroxidase exposure rats tissues |
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